Journal: bioRxiv

Article Title: Collaboration between the Fab and Fc contribute to maximal protection against SARS-CoV-2 in nonhuman primates following NVX-CoV2373 subunit vaccine with Matrix-M ™ vaccination

doi: 10.1101/2021.02.05.429759

Figure Lengend Snippet: Serum was collected day 21 and day 31/32 after the first dose of NVX-CoV2373, and profiled for the anti-NVX-CoV2373 antibody response. Luminex was used to quantify the ( A ) antibody isotypes (IgG1, IgA, and IgM) and ( B ) FcR binding (FcγRIIA-1, FcγRIIA-2, FcγRIIIA) for the anti-NVX-CoV2373 antibody response. ( C ) The functional anti-NVX-CoV2373-specific antibody responses for antibody-dependent cellular phagocytosis, antibody-dependent neutrophil phagocytosis, antibody-dependent complement deposition, and antibody-dependent NK degranulation (measured by CD107%). A two-way ANOVA with Tukey correction for multiple comparison was used to compare antibody levels between groups. Not significant (ns), *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

Article Snippet: The immune complexes were then incubated with lyophilized guinea pig complement (Cedarlane) and diluted in gelatin veronal buffer with calcium and magnesium (Boston Bioproducts) for 30 minutes.

Techniques: Luminex, Binding Assay, Functional Assay, Comparison

Journal: bioRxiv

Article Title: Molecular basis for protection and cross-protection by human antibodies targeting the parainfluenza virus hemagglutinin-neuraminidase protein

doi: 10.64898/2026.03.03.709347

Figure Lengend Snippet: Antibody binding to virally infected cells and associated complement-dependent cytotoxicity assay. (A) LLC-MK2 cells were infected with PIV3 and stained with PE-conjugated PIV3 HN-specific mAbs 5217-2 and 5217-9. The anti-F mAb PIA174 was used as a positive control and a mAb against Streptococcus pneumoniae , PhtD3, was used as a negative control. Both the HN-specific mAbs and anti-F mAbs showed binding to PIV3-infected cells. The data represents three technical replicates from one experiment and are representative of two biological replicates. (B) NeutrAvidin beads were coated with biotinylated PIV3 HN protein and incubated with individual mAbs. Guinea pig complement C3 was then added to the immune complexes. Complement deposition was analyzed using the Cytek Aurora flow cytometer and measured relative to C3 deposition in the absence of antibody. Data points are the average of two technical replicates from one experiment and are representative of two biological replicates. The data is presented using mean values +/- standard deviation.

Article Snippet: Guinea pig complement (MP Biomedicals, Cat: 64283) was diluted 1:50 in R-10 buffer (RPMI-1640+10% FBS).

Techniques: Binding Assay, Infection, CDC Assay, Staining, Positive Control, Negative Control, Incubation, Flow Cytometry, Standard Deviation